FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21846543
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA54000.011.0495141
AACGGGA50100.010.3909178
ACGGGAT39100.09.78659
AAACGGG60850.09.68867
GAAACGG61250.09.5526396
GGAAACG62900.09.3374225
CGGGATT37350.08.57724110
ACCGCGC17800.07.62471131
CACCGCG19300.07.032164630
CGGGATA9501.8189894E-127.025437410
ACGCCCG15600.06.98866134
ACAGGCG83550.06.87008218
CCGCGCC19750.06.75928332
CCGAGTA36250.06.69217542
CCCAAAG224850.06.59452441
CGGGAAT23550.06.518293410
GTTGGAA116800.06.4214912
CGCCCGG27050.06.333531435
AACGGGT13850.06.26455938
GCGTGAG56300.06.243575622
Produced by FastQC (version 0.11.5)