FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17297487
Sequences flagged as poor quality0
Sequence length25-288
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATC19309.190135E-766760.414265-269
CCGGACG7055.5181263E-636552.51255-259
CGTATCT32401.553965E-519883.889265-269
CGGACGG8052.7338821E-516848.33255-259
CGGAAGG35653.1355543E-514456.954260-264
GTGATCT228800.011262.902270-274
GTCTCGT26101.3108429E-47594.9067260-264
TCTCGTA20251.3758216E-47485.699260-264
GTGGTGT175357.586105E-57348.024265-269
CTCGTAT18601.7923085E-46597.419260-264
GGTTCGG14252.1791215E-46027.9575250-254
TGATCTC241001.4329841E-45346.3735270-274
AGTCTCG61803.3918617E-44633.139260-264
GGACGGA24304.7341024E-44078.7463255-259
GATCTCA218303.527049E-43934.8787270-274
CCGCGTT5705.532489E-43831.329240-244
GTTCGGA13256.4311194E-43536.1262250-254
GTATCTA123155.6119275E-43487.5515265-269
TCTCGGA27257.9678994E-43152.2349250-254
GACGGAA27708.8009494E-43000.992255-259
Produced by FastQC (version 0.11.5)