FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19046466
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN192830.10124187867712572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA43850.012.6905641
AACGGGA43500.010.227288
ACGGGAT34500.010.0582289
CGGGATT32900.09.80360510
AAACGGG53050.09.7280927
GAAACGG54900.09.6027966
GGAAACG55550.09.0900875
GTTGGAA95650.06.8859922
AACGGGT10400.06.844418
CGGGATA6751.6569138E-66.261285310
CCCGGAT6801.8582177E-66.2194961
CGGGAAT20250.06.15143810
GCGTGAG49300.06.091487422
ACCGCGC14450.05.85022831
CGTGAGC50750.05.82982523
ACAGGCG69100.05.730103518
CCCAAGT79600.05.62073951
CGCGCCC12301.72804E-105.606987533
GATTTCG21100.05.58752735
CCCATAA52500.05.5118141
Produced by FastQC (version 0.11.5)