FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17034184
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1317090.7732040466393929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA38300.010.6413381
ACGGGAT27250.09.1431699
AACGGGA37500.09.0761488
AAACGGG44500.08.94817357
GAAACGG46750.08.5651226
GGAAACG48000.08.34207255
CGGGATT27150.08.27579410
GCCGTCT18650.07.549568745
CGTAGAC4250.0099693717.454088745
AACGGGT9551.8189894E-126.9881038
CGTGAGC41800.06.439799323
ACAGGCG61650.06.3868518
GCGTGAG41450.06.333144722
CCCAAAG179800.06.01991751
CGGGATA6352.7391325E-55.955706610
ACGAATC16300.05.868902726
ACCGCGC14050.05.859015531
CGAATCT17100.05.854743527
CACCGCG14450.05.850778630
ACACGTC16150.05.78519737
Produced by FastQC (version 0.11.5)