FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24783113
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN500900.20211343102862017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA55600.010.5743231
CGTCCGA1955.4633926E-410.2676496
GAAACGG65800.09.0609146
AAACGGG65600.08.8850117
ACGGGAT37600.08.7566129
AACGGGA51350.08.7079898
GGAAACG68450.08.417645
CGGGATT35800.08.14065410
AACGGGT15750.07.3448868
CGGGAAT24350.06.39543610
GCCACCG59800.06.064294328
GATTTCG28300.06.0529675
CCCTAAA73000.05.91837261
CCGAGTA43600.05.818872
CACCGCG23400.05.79974230
CCCAAAG271250.05.79640051
GTTGGAA126750.05.7941042
ACCGCGC23050.05.791321831
ACAGGCG97000.05.77952218
CCCTAAT48250.05.6771611
Produced by FastQC (version 0.11.5)