FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20222510
Sequences flagged as poor quality0
Sequence length35-51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA50200.010.8671871
ACGGGAT34800.010.4210569
AAACGGG55400.09.5179187
CGGGATT34350.09.32693910
AACGGGA47550.09.1708368
GAAACGG59800.08.8176036
GGAAACG60800.08.599395
ACCGCGC21050.07.716422631
AACGGGT12450.07.50556338
CGGGATA8451.72804E-106.845733610
ACAGGCG91900.06.70634518
CCGCGCC24600.06.69332532
GCGTGAG59900.06.53730722
CCGAGTA43000.06.52170662
CACCGCG24750.06.472970530
CGCGCCC17400.06.393902333
CGCGCCT10104.0017767E-116.388838333
CGAGTAG44100.06.35903363
CCCAAAG226750.06.31420141
CGTGAGC63050.06.21091623
Produced by FastQC (version 0.11.5)