FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34979164
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA73150.011.9428411
AACGGGA73250.010.3253318
ACGGGAT55550.09.8510079
AAACGGG90100.09.7769427
CGGGATT50650.09.44264310
GAAACGG91700.09.3637686
GGAAACG94600.09.1943845
ACCGCGC26450.07.99058231
CGCGCCC22650.07.46434633
GCGTGAG84150.07.402147322
CGGGAAT35950.07.177837410
CCCATAA102550.07.1715691
CCGCGCC31550.07.1220432
CACCGCG29350.07.049350730
ACAGGCG114900.06.872719318
CGTGAGC90450.06.8619923
GCCACCG78000.06.56009428
CCCAAAG365300.06.50344371
CCCTAAA105600.06.50013971
CCCAAAA180750.06.23890071
Produced by FastQC (version 0.11.5)