FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33375588
Sequences flagged as poor quality0
Sequence length35-51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA66850.011.4692331
AACGGGA63850.09.6507688
ACGGGAT50000.09.4765019
AAACGGG82100.09.2667187
GAAACGG88050.08.5647236
CGGGATT50400.08.47447910
GGAAACG89150.08.4092785
CGGGATA13200.06.74106310
CAAAGCG52750.06.2956581
CACCGCG34850.06.000655730
CGGGAAT29500.05.95727210
ACCGCGC30550.05.898694531
AACGGGT20000.05.89506868
GTTGGAA156550.05.86942242
AAGCGGC25900.05.840943
AAAGCGG32700.05.64714242
CGCGCCC26200.05.34921533
GATCGCG10704.2325805E-45.26716945
GCGTGAG89650.05.161418422
TATCCGC8752.3577339E-55.08469536
Produced by FastQC (version 0.11.5)