FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130439531
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13562131.0397254494881616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG38550.010.23527545
GATCGGA32600.09.086371
ATCGGAA51000.05.84401042
CGTATGC49200.05.50444843
Produced by FastQC (version 0.11.5)