FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93438107
Sequences flagged as poor quality0
Sequence length35-51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8947750.9576125081386762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG35450.013.18928345
TACGGCG5601.8189894E-129.5302836
GATCGGA19150.07.65800431
CGTATGC44500.06.997616343
ACGAGCT44500.06.896744329
GTCACGA42600.06.890740426
CTCGTAT42550.06.80524741
ACGACGC8308.094503E-106.70906541
CACGAGC47750.06.70665828
TCTCGTA44600.06.536133340
CGACGCT7857.582639E-86.27362742
ACGGCGA9704.2418833E-95.96056277
TGCCGTC41600.05.96030145
CGGCGAC10151.9244908E-95.9154098
CGAGCTG52750.05.86029530
TCGTATG50100.05.808597642
CACGACG9803.2567186E-85.67673240
AGTCACG59350.05.358125725
CGGAAGA52350.05.2579221
ACACGTC53650.05.222539410
Produced by FastQC (version 0.11.5)