FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19639385
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGG3409.31829E-611.38287945
CGTTGGA46750.010.47748851
ACGGGAT32750.09.37396059
AACGGGA45100.09.1746978
AAACGGG57750.09.0140347
GAAACGG57600.08.8443996
GGAAACG61450.08.2178925
CGGGATT30650.08.05652610
AACGGGT12650.07.561948
GCGTGAG53300.07.2625222
AATCGCC8502.0545958E-67.036689345
ACAGGCG78500.06.99994818
CGTGAGC55900.06.885123723
CCCAAAG206600.06.8755351
CACCGCG18600.06.69854130
GCCACCG50200.06.692345628
ACCGCGC17250.06.577866631
CGAAACG4404.1929097E-46.572755
CGGGATA8804.474714E-106.572733410
GATCGCG8206.676048E-56.43599745
Produced by FastQC (version 0.11.5)