FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21310577
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7684573.605988706922389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA5650.015.379961
CCGTCTT31200.010.19381945
ATCGGAA8450.010.0050852
CGTTGGA37650.09.7646791
TATGCCG32300.08.75258745
TCGGAAG9700.08.0273313
GCCGTCT29000.07.79885745
AACGGGA38550.07.7321328
AAACGGG47600.07.5707997
CGTCTTC25500.07.483462345
ACGGGAT29400.07.1122989
GAAACGG50600.07.03421746
TGCCGTC30650.06.951071343
CCCGTAA8755.2750693E-116.87534861
GATTGCG8259.302879E-66.853541445
GGAAACG52100.06.70360235
ACACGTC33650.06.610916
CGGGATT27650.06.59702510
CGGAAGA15650.06.54911331
ATGCCGT32550.06.395255642
Produced by FastQC (version 0.11.5)