FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27101804
Sequences flagged as poor quality0
Sequence length35-51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN294375210.861830452319705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA10500.026.7316551
ATCGGAA13600.020.1267592
TATGCCG17650.017.40348445
TCGGAAG15450.015.5548593
ATGCCGT18550.011.47847345
CGGAAGA21350.010.3180434
CGTTGGA56550.010.0844451
TCTCGTA20050.09.87957240
CGTATGC21250.09.37250843
CTCGTAT21200.09.14130541
TCGTATG22750.08.7552142
ACGGGAT39650.08.6414279
AACGGGA51800.08.547388
AAACGGG64000.08.5171627
GAAACGG69800.08.0327426
CGGGATT37550.07.998927610
GGAAACG69350.07.7640215
ATCTCGT25950.07.547049539
GCGTGAG67900.06.91497622
CCCAAAG261050.06.7669681
Produced by FastQC (version 0.11.5)