FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26516490
Sequences flagged as poor quality0
Sequence length35-51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN882423333.2782845693378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA18700.035.244231
ATCGGAA21900.028.0337922
TCGGAAG20300.025.4197243
CGGAAGA35050.015.6908621
GCCGTCT195900.011.63124145
GAAGAGC105150.010.1852961
AGAGCAC151000.09.3396212
CGTTGGA41200.09.2954221
AAGAGCA165500.08.6103311
GTCTTCT192050.08.45384645
TCGGAAA11900.08.4109373
AAACGGG47800.08.1883467
CGTCTTC148200.08.13412145
GAGCACA172500.08.123263
GAAACGG51000.07.97989656
GCACACG175950.07.9501495
TGCCGTC221300.07.903237345
CGGGATT28100.07.83497910
AACGGGA38300.07.78067838
ACGGGAT29000.07.668519
Produced by FastQC (version 0.11.5)