FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37078149
Sequences flagged as poor quality0
Sequence length35-51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12849073.4654022238273003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA12450.020.7509421
ATCGGAA17850.014.8351862
TCGGAAG20750.010.7231513
CGTCTTC49700.010.34134845
CGTTGGA80850.010.3024561
TATGCCG51300.010.0188145
AACGGGA81050.08.9759298
AAACGGG98100.08.7312377
CGGAAGA27400.08.2827594
ACGGGAT58850.08.2412129
GAAACGG104450.08.2218176
GGAAACG106150.07.85955145
CGGGATT57000.07.14261610
CCGTCTT52500.07.107584544
GCCGTCT47750.07.097341543
ACACGTC46600.06.92258935
CGTCCGA3907.680802E-46.84550676
TGCCGTC48550.06.82404942
CGGGAAT37600.06.686081410
ATGCCGT50400.06.497131341
Produced by FastQC (version 0.11.5)