FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18750991
Sequences flagged as poor quality0
Sequence length35-51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6403583.415062169247481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA4600.013.5486961
CGGAAGA12450.011.6209411
CGTTGGA37600.010.1229131
TATGCCG28200.09.73337245
AAACGGG44900.09.4117377
AACGGGA36750.09.2596938
CGGGATT24450.08.91476110
ACGGGAT27500.08.815699
GAAACGG47350.08.7368626
CGTCTTC25700.08.32223245
CACCGCG15300.08.28782830
GGAAACG47500.08.1942075
ACCGCGC14950.07.73783431
CGCGCCC10950.07.72023233
GCCACCG38750.07.52061328
AACGGGT10700.07.2752318
CCGCGCC14850.07.19083432
GCGTGAG42500.07.11804122
CGTGAGC42900.07.051671523
ACAGGCG56800.06.970831418
Produced by FastQC (version 0.11.5)