FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22317606
Sequences flagged as poor quality0
Sequence length35-51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2688511.204658779261539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA43300.011.3082061
ACGGGAT30950.09.7023729
AACGGGA43150.09.2273498
AAACGGG53450.09.1970667
GAAACGG57550.08.6577986
CGGGATT29200.08.60794310
GATCGGA4702.6815542E-88.5238061
GGAAACG55650.08.3938055
GCCGTCT39600.07.871495745
CGTCTTC38150.07.520734345
AACGGGT13100.07.30132878
CCGTCTT40950.07.179496345
TGCCGTC43550.06.994877345
CGGAAGA15300.06.9824651
CCTTCGA9709.640644E-116.4245871
CCCATAA60400.06.04318431
CGGGAAT19300.05.99307710
GTCGGGA8659.141149E-85.9144797
CCCAAGT89450.05.8969431
CGGGATA8801.2951023E-75.813650610
Produced by FastQC (version 0.11.5)