FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23484087
Sequences flagged as poor quality0
Sequence length35-51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1113823747.42886960008281No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGCTAACGAATCTCGTATGCCG362900.15453017185637236TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA17050.033.165291
CGGAAGA62950.031.7935091
ATCGGAA17400.030.934242
TCGGAAG16850.028.2445373
TATGCCG193400.019.71001645
GAAGAGC109300.019.1261833
AGAGCAC135600.017.1518785
GGAAGAG124450.016.924992
AAGAGCA140600.015.8166384
GAGCACA153800.015.0932886
ACACGTC162600.014.33089910
GCACACG161450.014.1851428
CACACGT166950.013.7710439
CACGTCT171150.013.62791711
ACGTCTG173600.013.61490212
CGTCTGA174050.013.56692413
AGCACAC174850.013.1616147
GTATGCC209500.013.02970744
AGTCACG195600.012.61929525
GTCACGC197450.012.52517726
Produced by FastQC (version 0.11.5)