FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23626152
Sequences flagged as poor quality0
Sequence length35-51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN353100.14945302984590972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA43700.011.6184511
CGTCCGA2302.3058006E-49.6709826
AAACGGG58600.09.1477467
GAAACGG59700.08.9420136
AACGGGA46200.08.85876758
GGAAACG60100.08.62342555
ACGGGAT36050.08.3297069
CGGGATT35450.08.09429810
AACGGGT13200.08.088448
GATCGCG7602.999259E-77.94450145
GTCGCTC5550.0011049677.039324345
CAAAGCG37400.06.49007561
ATCGCGC5951.004576E-56.35648338
GGCTAAT122350.05.91526561
AAGCGGC17700.05.907093
ACCGCGC16100.05.80300731
CGTATGC8607.5457623E-45.78177145
GTTGGAA110950.05.71611552
CGGGAAT23500.05.67922610
CGAAACG5300.0039122725.45589265
Produced by FastQC (version 0.11.5)