FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24059385
Sequences flagged as poor quality0
Sequence length35-51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN300230.12478706334347284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA50300.012.128631
ACGGGAT37850.09.9307189
AACGGGA50400.09.7525458
AAACGGG63400.09.7172657
GAAACGG66450.09.1708786
CGGGATT37400.08.86101710
GGAAACG65950.08.8357175
AACGGGT14850.07.7881298
CGGGATA8057.2759576E-127.45996510
CGAAACG4405.494925E-57.0766995
CGCCCGG25250.06.254935335
GGCTAAT114700.06.13412761
CGGGAAT24000.06.11648110
GTTGGAA117700.06.06826732
ACGGGTT14000.06.03692059
ACCGCGC16500.05.79680531
CCCGGAT7356.939066E-65.7556641
GCTAGAC48550.05.681107529
ACTGCGC18200.05.62199731
ACGCCCG13153.092282E-115.582171434
Produced by FastQC (version 0.11.5)