FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13630586
Sequences flagged as poor quality0
Sequence length25-299
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAAG17751.4041589E-521603.684265-269
AGGGGGG55301.3629435E-517335.688270-272
CCGAAGG19603.595375E-513974.6875265-269
TCGAACC13506.3353014E-510924.94260-264
CGAAGGG21457.3817944E-59931.765265-269
GGGGGGA80208.599742E-57968.941270-272
CGAACCA13052.0946638E-46121.7334260-264
ACGCTAC13354.455418E-44224.1177255-259
CCAACCG14705.401994E-43836.1885260-264
ATCGTAG14805.475734E-43810.2683255-259
CAACCGA13356.194526E-43590.5260-264
GGGGGAA134204.8155215E-43571.7715270-272
GAATAAA323804.6728435E-42960.6655270-272
GACGAAG20550.00105563752744.1348255-259
GTCGGTT9000.00128845132506.3098250-254
AAAAGGC167550.00125095172288.6626265-269
CGCTACC13450.00157416592262.7334255-259
TCGTAGG13450.00157416592262.7334255-259
AACCGAA23100.00235362191844.4706260-264
CGTAGGG12300.00252155031791.7269255-259
Produced by FastQC (version 0.11.5)