FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135597512
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4908190.3619675558648893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG41950.011.50779845
CGTATGC49200.06.006669543
CTCGTAT51550.05.87985541
TCTCGTA54750.05.40892340
TCGTATG63550.05.145669542
Produced by FastQC (version 0.11.5)