FastQCFastQC Report
Thu 9 May 2024
EGAF00005189291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30120318
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2563385885.10487173475393No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTAGAATCTCGTATGCC1197990.39773484463211844TruSeq Adapter, Index 20 (97% over 34bp)

[OK]Adapter Content

Adapter graph