FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30120318
Sequences flagged as poor quality0
Sequence length35-51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2563385885.10487173475393No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTAGAATCTCGTATGCCG1197200.39747256320467794TruSeq Adapter, Index 20 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG310950.043.85589645
GATCGGA142550.043.6855351
ATCGGAA141100.042.8603632
TCGGAAG130150.042.4064033
CGGAAGC42150.036.248194
TGCCGTC66450.034.4741245
GACACGT25700.033.4461639
AGACACG26550.032.2918668
CGGAAGG19700.031.2034534
GTATGCC331150.030.8178744
GGAAGAG217850.030.2859042
ACACGTC252050.029.26144410
GGAAGAC38900.028.9087335
AGAGCAC226250.028.7888665
CACACGT226850.028.781769
AAGCACG19300.028.7299027
GAGCACA229050.028.6414246
CACGTCT282700.028.3496811
CGTAACT5300.028.15019216
CGGAAGA227700.028.1225931
Produced by FastQC (version 0.11.5)