FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11398160
Sequences flagged as poor quality0
Sequence length25-299
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTC9001.4160378E-662102.78275-279
GCTACGT14957.814457E-628039.717270-274
ACGTACG4751.5777105E-522062.83270-274
CGTACGT4151.716131E-521265.379270-274
TACGTCT18652.0268346E-517981.502275-279
CTACGTA17054.7430687E-512293.073270-274
CGTCTGA17056.0981896E-510927.177275-279
TACGTAC14708.310431E-59505.527270-274
ACGTCTG26508.593271E-59039.219275-279
TCTGAGC136551.0865599E-46139.7847280-284
GTACAGC56405.189531E-43716.2568265-269
AGCTACG15205.855625E-43677.1382265-269
TCGATCG4707.162206E-43365.6665255-259
TTAGCTT129555.8674806E-43235.768270-274
TTGATGC87606.7067065E-43190.2114280-284
TTGGAGA176205.427164E-43172.1057275-279
CCGCGAG4759.543256E-42917.399250-254
AACCCCA150207.886959E-42790.9038270-274
CTGAGCA157708.6942164E-42658.1724280-284
TTCGGCA11900.00131669162472.026255-259
Produced by FastQC (version 0.11.5)