FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21794764
Sequences flagged as poor quality0
Sequence length35-51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2037530.9348713296459645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA56450.010.6249851
ACGGGAT41200.010.0420849
CGGGATT38750.09.64358810
AAACGGG63950.09.4263577
AACGGGA55100.09.3255728
GAAACGG68800.08.891186
GGAAACG70300.08.4480025
ACCGCGC14200.07.67566231
CCGAGTA24250.07.06348852
CACCGCG15450.07.0546730
CCGCGCC16200.07.00282632
CCCGAGT36850.06.77673531
CGCGCCC11300.06.69301233
GATTTCG26750.06.48587945
CCCTAAA62950.06.41095641
AACGGGT12000.06.30249938
TGCCGTC14103.2832759E-96.300616345
GCCGTCT14354.8476068E-96.190849345
GCCCGAT4350.00257323126.1508051
GTTGGAA118550.06.0984872
Produced by FastQC (version 0.11.5)