FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24732983
Sequences flagged as poor quality0
Sequence length35-51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4371471.7674657359365025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT18250.014.17356345
TATGCCG14550.012.02718844
ATCGGAA11000.011.5493761
TCGGAAG11000.010.719352
AACGGGA52750.010.036017
GAAACGG66050.09.7661375
CGGGATT36450.09.7651149
ACGGGAT39900.09.7571038
AAACGGG65850.09.6606856
GGAAACG68800.09.248344
CTCGTAT18300.08.8750640
CGTATGC17800.08.8029442
TCTCGTA18150.08.58025939
ACACGTC18150.07.7218049
TCGTATG20850.07.68986441
CGTCTGA19300.07.607632612
CGGAAGA15850.07.2989323
ACCGCGC18450.07.234669730
ATCTCGT21150.07.15269238
CGGGATA9001.4551915E-116.92102439
Produced by FastQC (version 0.11.5)