FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19073046
Sequences flagged as poor quality0
Sequence length35-51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN314080.16467217664131886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA44600.09.3843911
TCGTTCG2607.814269E-48.5597344
AACGGGA41100.07.90407668
AAACGGG52600.07.8680957
ACGGGAT31000.07.75175339
GAAACGG54650.07.45074846
GGAAACG55450.07.18287755
CGGGATT27850.06.71103710
GGTCGAT3650.00286052826.7093881
CGGGATA8103.43789E-96.592676610
CCCAAAA93650.06.39480921
ACAGGCG81550.06.248251418
GTCGATT4658.145429E-46.22232342
CCCAAGT94600.06.14232061
CGTGAGC54950.05.952680623
GCGTGAG55200.05.8854122
CCCTAAA56250.05.8576351
CCGAGTA36650.05.8298772
AACGGGT13501.8189894E-125.7686538
CACCGCG19550.05.691356730
Produced by FastQC (version 0.11.5)