FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9422395
Sequences flagged as poor quality0
Sequence length25-298
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTC10352.6749967E-645184.207275-279
GCTACGT14553.700442E-513774.862270-274
TACGTCT18754.3893648E-512470.842275-279
ACGTACG6105.2961186E-512105.002270-274
CGTACGT6208.0946935E-59838.497270-274
CTACGTA16901.3074589E-47546.906270-274
CGACGCG1551.5497053E-47241.134245-249
CGTGGAC15101.726956E-46636.5645260-264
TACGTAC14551.8501187E-46428.269270-274
TCTGAGC113451.07135245E-46183.207280-281
CGTCTGA20002.197283E-45845.707275-279
TTTACCA123251.264437E-45691.5605280-281
ACGTCTG28852.4938813E-45403.3115275-279
TCGATGT21853.6158762E-44586.367260-264
ATCGATC12304.0921918E-44387.0225255-259
CGATGTA14154.999083E-43965.9922260-264
TCGATCG7205.720811E-43747.248255-259
GTACAGC51106.08499E-43431.922265-269
CTGAGCA137504.7208654E-43401.1387280-281
GTGGCCC63707.0917886E-43146.3774270-274
Produced by FastQC (version 0.11.5)