FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19366558
Sequences flagged as poor quality0
Sequence length35-51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA46400.010.1286741
ACGGGAT32100.09.9298539
AACGGGA42350.09.8695578
CGGGATT28900.09.56411310
AAACGGG52150.09.4644237
GAAACGG54450.08.7788196
GGAAACG54250.08.56528955
CCCCGAA6501.9408617E-97.5387171
CCCGTAA7752.1827873E-117.4723931
ACGCCCG10800.06.999471734
CCCGATA4852.5960566E-56.88870531
CGTTCGA3600.00246218276.80578571
AGTGTCG5250.0074290646.803469745
GCCGTTA3300.0080233246.749541
CCCGAAC4303.1210255E-46.73384331
CGGGAAT19150.06.401986610
CGCCCGG20200.06.38390935
AACGGGT11950.06.3256378
CCCAAAG188450.06.2878711
ACAGGCG53850.06.234350718
Produced by FastQC (version 0.11.5)