FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14477097
Sequences flagged as poor quality0
Sequence length35-51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2231701.5415383346536948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7453.6379788E-1210.21599445
CCGACAA7303.5288394E-107.322608531
CTCGTAT7809.242285E-96.614271641
CGTATGC8809.300493E-96.293626343
CGACAAC8152.6191628E-86.2880732
TCTCGTA8051.2391865E-76.11834940
CCCGACA10004.9847586E-85.566529330
TCGTATG10002.2166205E-75.414247542
ATCTCGT11351.2734563E-75.12366839
ACGGGTT10350.00195949135.01377845
Produced by FastQC (version 0.11.5)