FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13067466
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1090780.8347295489423886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5300.015.16378245
CTCGTAT6209.094947E-128.65164341
TCTCGTA6054.9112714E-118.48536340
CGTATGC7259.335054E-96.972315343
GATCGGA3907.6623244E-46.8468681
TCGTATG7706.830305E-96.711498342
ATCTCGT9509.597534E-85.635989739
CATCTCG11952.6138878E-95.41139438
Produced by FastQC (version 0.11.5)