FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15789164
Sequences flagged as poor quality0
Sequence length35-51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN193314812.243510802725211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8100.029.1586671
TATGCCG21300.025.94620545
ATCGGAA9800.023.6068172
TCGGAAG10900.018.1618143
TGCCGTC15200.017.48020645
CGGAAGA17400.015.6227471
ACCGGAT23050.015.15194229
CGGATTG23200.015.05392231
CGTATGC24600.014.72693443
CACCGGA24050.014.70696928
CGTTGGA29300.014.6769731
TCACCGG23650.014.57936327
ACACGTC20800.014.54315810
CCGGATT24500.014.52748130
CTCGTAT24050.014.44391341
GTCACCG24600.014.28762326
CGTCTGA22750.014.17652313
TCTCGTA24300.013.91625440
TCGTATG25600.013.72880542
ATCTCGT26100.012.87113239
Produced by FastQC (version 0.11.5)