FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17440504
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN303942517.42739200656128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG40250.032.60223445
GATCGGA8900.027.5395181
CGGAAGA29100.025.2682191
ATCGGAA10250.023.6591452
CGTATGC42800.019.90086743
ACACGTC38800.019.78035510
TCGGAAG10600.019.5179583
CGTCTGA40700.019.23948513
CTCGTAT43100.019.0671441
TCGTATG44550.018.73536742
TCTCGTA41900.018.53607640
ACGTCTG43000.018.46906912
GTATGCC52800.017.68448444
CACGTCT43700.017.66441511
ATCTCGT44950.017.52594839
GCACACG43900.017.3810628
CATCTCG46600.016.71413838
CACACGT46550.016.439389
ATGCCGT25300.013.90541445
TGCCGTC19900.013.61263145
Produced by FastQC (version 0.11.5)