FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26559451
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1988860.748833249602938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7250.016.53045745
CGTTGGA57400.012.3004991
TCGCGAC1350.004364535711.53546435
AAACGGG68200.010.24163157
AACGGGA56500.09.9608018
ACGGGAT43350.09.954899
GAAACGG71850.09.597556
GGAAACG70700.09.502485
CGGGATT41350.09.41426510
ACCGGAT8950.08.45161929
CTCGTAT11100.07.62412141
CGGATTG11800.07.541145331
TCTCGTA11400.07.416922640
CGTATGC11750.07.017903343
AACGGGT17500.06.99112138
CCGGATT11400.06.63484630
GTTGGAA133700.06.4912272
GCGTGAG64600.06.473956622
CCCAAAG277400.06.47148471
ACAGGCG93850.06.399826518
Produced by FastQC (version 0.11.5)