FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29563060
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA58150.07.46431831
CCCGTAA11750.07.3880871
ACCGCGC24550.07.340015431
CCCGAAA10650.07.31515741
CGCGCCC21200.06.92589533
CACCGCG28450.06.802987630
GCGTGAG75500.06.718528322
CGTGAGC81300.06.51260923
GCCACCG71100.06.44557728
CCCAAAG311050.06.440471
ACAGGCG109550.06.37683218
CCCATAA81050.06.2616261
CCGCGCC29600.06.23808532
CCCGGAT11301.8189894E-116.10644631
AACGGGA55150.06.0910378
TATCCAC119500.06.087546
CCCGATT10304.2200554E-106.0509851
CGTATGC8254.9652543E-45.97413745
CCAAATA193350.05.9518451
GAAACGG70700.05.91562186
Produced by FastQC (version 0.11.5)