FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38320119
Sequences flagged as poor quality0
Sequence length35-51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC26050.08.3043831
CGTTGGA78950.08.2608461
GCGTGAG84950.07.695937622
CGGGATT49700.07.69565710
CACCGCG29500.07.4843730
AAACGGG88000.07.4038577
CCGCGCC29500.07.333199532
AACGGGA73350.07.27584848
ACGGGAT54300.07.08471169
CGCCCGG37350.07.04576835
CGCGCCC20950.07.025854633
CGTGAGC91450.07.003045623
GAAACGG91100.06.8590036
GCCACCG75950.06.675635328
CGTATGC12207.9835445E-96.66132645
ACAGGCG122950.06.62049218
CCCTAAA115600.06.52756171
GGAAACG96050.06.50552035
CGCGCCT12100.06.45092433
CCCAAAG386800.06.3762071
Produced by FastQC (version 0.11.5)