FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21686147
Sequences flagged as poor quality0
Sequence length35-51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC12650.030.7569753
GCGCGAT13050.029.644072
GGCGCGA13150.028.7536241
ACCGCGC26150.027.1324121
CGCGCCC19800.026.4998173
CCGCGCC27150.025.7133622
ACCGCAC20200.024.333821
GCGATCT25000.023.567094
GACGCGA1450.023.008731
CGCGATT1550.022.9500893
CGCCCGG28950.021.9603585
ACGCGAT1750.021.5978222
CCGCACC17950.021.5518172
ACCGTGC25450.021.4989241
CTCACGC48100.021.216013
GCTCACG48300.021.1283042
GGGCGAC22100.020.7192916
GGCGCAA9750.020.3027461
CCCGGCC49800.019.8633127
ACTGCGC23300.019.759861
Produced by FastQC (version 0.11.5)