FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38622212
Sequences flagged as poor quality0
Sequence length35-51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4256521.1020912007836319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC25050.019.363223
GCGCGAT23900.019.2710442
GGCGCGA24900.019.0390871
ACCGCGC42300.018.5211581
CCGCGCC47350.016.7757132
CGCGCCC35050.016.5049483
CTCACGC98800.016.349653
GCTCACG99700.016.3361112
ACCGCAC39900.016.1209551
CGCCTGT111950.014.2095027
GCGATCT47850.014.0892464
ACCGTGC47150.013.9253521
ACTGCGC37950.013.8996041
GCGCAAT19950.013.8296612
CCGCACC36550.013.5753252
GGATCAC206250.013.47250145
GGCGCAA19650.013.3655021
CGCCCGG56150.013.0360135
CGCAATC23300.012.7006073
CGGATCA59450.012.49751144
Produced by FastQC (version 0.11.5)