FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24406880
Sequences flagged as poor quality0
Sequence length35-51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13417375.4973720524704515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA7200.015.7778391
CGGAAGA21700.011.0859741
ATCGGAA11250.010.8798392
TATGCCG44100.010.21915945
CGTTGGA48750.09.5038271
TCGGAAG13000.09.2431563
TGCCGTC40200.08.86417145
AACGGGA45600.08.2942268
AAACGGG57150.07.98046977
GCCGTCT37800.07.948234645
ACGGGAT33850.07.6240619
CGGGATT32500.07.59847910
GAAACGG62450.07.5882526
GGAAACG62800.07.40423635
ACCGCGC22250.07.000248431
GCGTGAG63600.06.927147422
CCCAAAG245350.06.78179071
ACACGTC40350.06.726719410
CGTGAGC66500.06.524709723
CGTGATA8551.5697879E-96.513041
Produced by FastQC (version 0.11.5)