FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29278790
Sequences flagged as poor quality0
Sequence length35-51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN881472030.106162174051594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA13350.030.5274011
ATCGGAA16600.024.9297622
TCGGAAG17200.021.600573
CGGAAGA25550.013.4956434
TATGCCG62050.011.61309545
CGTTGGA52150.09.94996551
AACGGGA47900.09.6588278
AAACGGG58900.09.44107157
ACGGGAT35550.09.3226879
GAAACGG63100.08.8832086
GGAAACG63700.08.659845
CGGGATT36800.08.15981610
CGTCCGA3003.5852814E-48.1558676
CGTCTGA46400.07.430312613
CGTATGC65650.07.36609743
ACACGTC44100.07.01084910
CTCGTAT66000.06.886601441
GTATGCC78550.06.866232444
TCTCGTA64500.06.764319440
TCGTATG69150.06.733777542
Produced by FastQC (version 0.11.5)