FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27537094
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5071301.8416249732088652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG7900.034.23314
ACGGCGA9850.027.6740235
CGACGCT9400.026.02340340
CGGCGAC11550.024.1744086
ATACGGC11500.023.5168273
CGACCAC13450.020.5946039
CACGACG12350.020.30352838
ACGACGC12350.020.14581539
GCGACCA14150.019.575868
GACGCTC13000.019.37532641
GGCGACC14500.018.6448947
ACCGAGA16600.016.6866214
TACACGA15900.016.16181236
GATACGG16500.016.1224162
ACACGAC15950.015.98566237
TGATACG16100.015.4388951
CACCGAG18750.015.00964713
CCACCGA18400.014.69296112
TATGCCG13000.013.55792645
CTACACG19650.013.40627135
Produced by FastQC (version 0.11.5)