FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35298304
Sequences flagged as poor quality0
Sequence length35-51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5145421.4576960978068523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG15850.010.4209345
GATCGGA9450.08.2412951
CTCGTAT18150.06.278291741
TCTCGTA16850.06.09145340
CGTATGC20400.05.404401343
ATCGGAA14902.0008883E-105.07126332
Produced by FastQC (version 0.11.5)