FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19629075
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG2600.021.3886996
CGACGCT2550.019.31231542
ACCGCGC20900.018.4618131
ACGGCGA3300.017.5257687
GCGCGAT11350.017.0531312
CGCGATC11750.016.4725993
GGCGCGA12400.015.8283441
ACGACGC3100.015.80528741
CCGCGCC24500.015.8002482
CACGACG3150.015.54085140
CGCGCCC19150.015.2189013
CGGCGAC4400.013.6498788
GCGATCT21850.012.9309944
ACCGCAC17950.012.1768831
GGCGACC5800.011.8891669
CGCGCCT9750.011.6371413
ACCGTGC21000.011.4704111
CACGCGA2351.9556082E-611.3890591
CCGCACC18050.010.8464152
GCGACCA5400.010.71019210
Produced by FastQC (version 0.11.5)