FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15958486
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN847831253.12729540885018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA77900.042.9047431
TCGGAAG74700.040.3844262
CGGAAGA65850.037.8730933
CGGACGA1400.031.7537983
CGTCCGA1500.031.11934315
ATCGGAG5950.030.4143981
CGGAAGC12200.028.0578633
TCGGAAA13100.027.8308982
ATCGGGA7300.027.5442391
ACGTCCG1700.027.45806314
TCGGACG1900.026.9109422
ATCGGAC2150.024.9392831
CGGAAAG11500.023.1940783
CGTCGGA1353.6379788E-1223.05136715
ACGTCGA602.6811092E-422.22795514
GCACGTC6400.021.53347610
ACACGAC3850.021.36164712
CACGTCG2150.020.67689313
CGGAAGG10900.020.3923473
CACGTCA7050.020.178313
Produced by FastQC (version 0.11.5)