FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11007862
Sequences flagged as poor quality0
Sequence length35-51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN210400219.113629876537335No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGCATACAATCTCGTATGCCGT360030.3270662368405418TruSeq Adapter, Index 22 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA27700.040.1834751
ATGCCGT75350.038.52193545
TCGGAAG28750.036.6786842
CGGAAGA28300.033.3277783
TATGCCG83100.024.70976444
CACACGT79300.022.8614658
ACACGTC81150.022.8062789
GCACACG80450.022.5623197
CGTCTGA86200.021.65069612
ACGTCTG87150.021.33811811
CACGTCT87250.021.23729310
CGTCGGA1056.904884E-821.1852115
GAAGAGC86500.021.1411672
AGAGCAC87850.020.7381044
GTATGCC93350.020.50871543
GAGCACA88900.020.4681435
CGTATGC93600.019.9249542
GTCACCG95150.019.47927525
GCACGTC5850.019.39250610
CCGCATA98600.018.79949229
Produced by FastQC (version 0.11.5)