FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10107008
Sequences flagged as poor quality0
Sequence length25-291
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGC13604.432768E-635100.27270-274
ACGCAAC8308.255045E-628756.848270-274
CGCAACA9751.5947679E-520983.016270-274
AACGCAA14951.785468E-519158.406270-274
AAACGCA21452.205342E-516691.035270-274
ATCTTAC74801.3408743E-46381.8667270-274
GGCCGAC10106.681219E-43458.3215255-259
CCAAACG17758.1784825E-43103.122265-269
GAGGCTA73208.987652E-42794.869265-269
GTTGTCT81400.00111138512513.322265-269
TATATTA191958.8295265E-42486.9167270-274
TACCGCC5900.00154711962289.88250-254
GCCGACA10400.00226997721886.3157255-259
CGCCGAA1800.00230936941880.8654240-244
AGGCTAT71400.00317549221542.8689265-269
GGCTATA51650.0032594461540.3796265-269
ATGCTAC65900.00364137771448.7515260-264
AGGGCAC70200.0041319761360.0103260-264
ACGCCGA2500.0044541911354.2231240-244
AAGAGAT162550.0044310611258.5936265-269
Produced by FastQC (version 0.11.5)