FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8990322
Sequences flagged as poor quality0
Sequence length25-299
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT3156.7043904E-510914.53255-259
ACCGGTC4301.2492976E-47995.528255-259
TTCTTCC233800.05882.082275
TGGGCGA28203.7885783E-44433.368265-269
TCACCGC15204.2025646E-44308.367260-264
TGAGGGC81003.4100123E-44244.5396270-274
GGGCGAG16454.4534015E-44180.0327265-269
GACAAAC88704.0891205E-43876.0732270-274
GCGGCAG18105.958992E-43618.0764260-264
GTACGTC6950.00112994592673.9856255-259
ACCGCCC10950.00171648782165.377260-264
CCGGTCA8800.00181144562111.8408255-259
CACCGCC17250.00211309571944.4762260-264
CGGCAGG18250.0023651491837.9294260-264
CACTAGA68250.00221874521831.8093265-269
GCCCCAC69650.00231069021794.9889265-269
GAGGGCA98850.0023694921739.0375270-274
CCGCTAC7950.0034632491530.8407250-254
TTGGGCG10900.00358641871501.9995260-264
TTACTTT232350.00280548211479.6975270-274
Produced by FastQC (version 0.11.5)