FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31191947
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA71900.010.4538561
ACGGGAT47850.09.3439879
AAACGGG81600.09.3229817
AACGGGA66350.09.051948
GAAACGG84000.08.9243036
CGGGATT44000.08.74619610
GGAAACG84900.08.725055
AACGGGT20800.07.4860648
CGGGATA13000.06.331172510
GATCGCG11508.4675594E-76.14208845
CCCATAA91900.06.04309371
CGGGAAT33000.05.729685310
GGATCCG9504.7349502E-45.576369345
GTTGGAA165050.05.5403132
CCCGTAA12552.746674E-105.50926261
ACCGCGC24700.05.493849331
CCCTAAA93250.05.4772451
ACGGGTT19100.05.47372069
CCCGAGT66400.05.44154741
CCGAGTA45400.05.43970162
Produced by FastQC (version 0.11.5)