FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22241302
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA49900.012.71351
AACGGGA48250.010.6966228
AAACGGG59800.010.3047287
GAAACGG60100.010.2902136
ACGGGAT36100.010.229519
CGGGATT32900.09.93968310
GGAAACG61800.09.5032775
CGTCCGA1900.00507738779.3667826
CGGGAAT23400.07.41531910
CGAAACG5151.0483E-67.3434415
GTTGGAA111500.06.90531162
CGGGATA10301.8189894E-126.69538510
ACGGGAA24300.06.3167969
TAGGCGT9251.4133548E-96.25308619
AACGGGT14850.06.14205038
GCGTGAG59050.05.952869422
CCCTAAA65200.05.83807041
CCCAAAG237900.05.8012031
ATAGGCG9255.8184014E-85.772131418
CGTGAGC61900.05.64285923
Produced by FastQC (version 0.11.5)